ABSTRACT
The present study aimed to assess the levels of some heavy metals [Cu, Fe, Pb, Zn and Cd] in muscles of wild and farmed Oreochromis niloticus as well as to evaluate the human hazard index associated with fish consumption
In addition, total protein, molecular weights and band counts of sarcoplasmic proteins were investigated with sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE] method. The obtained results revealed that the accumulation of Cu has the highest value in farmed Oreochromis niloticus compared to wild Oreochromis niloticus while the highest Fe, Pb, Zn and Cd concentrations were recorded in wild Oreochromis niloticus compared to farmed Oreochromis niloticus
The calculated hazard index [HI] indicated that all metals had low HI except Pb and Cd levels in both wild and farmed fish were higher than their permissible limits for fish as a human food
There was no significant [P> 0.05] difference between wild and farmed Oreochromis niloticus in total protein
Wild fish predominant farmed fish in the number of separated proteins
Wild fish muscle protein showed 12 protein bands, while farmed fish muscle proteins showed 11 protein bands. Also, these bands were differed in quantitative parameters. Wild fish had unique bands [MW. 198.13, 97.92, 56.77 and 29.75] while farmed fish had unique bands [MW. 121.62, 79.05 and 26,16]
The current data found that there are differences in electrophoretic pattern and heavy metals accumulation between wild and farmed Oreochromis niloticus